Abstract
Introduction: Accumulation of senescent cells contributes to the development of cancer, chronic inflammation and age-related pathologies. Aim of our study was to investigate the effect of eicosapentaenoic acid on cellular senescence, as eicosapentaenoic acid is one of the most important omega-3 fatty acids in human cells.
Material and methods. To induce senescence, MRC-5 human lung fibroblasts were incubated with 100 µM hydrogen peroxide/0.5 hour or 80 µM etoposide/1 hour. Eicosapentaenoic acid (50 µM) was added to the cells for 24 hours after the induction of senescence. Cell growth, activity of SA-bgalactosidase, p21 protein level and reactive oxygen species were determined on the 4th day after the induction of senescence. Investigation of caspase-3 protein level and horisontal gel electrophoresis were performed immediately after the end of incubation of cells with eicosapentaenoic acid or on the 1st, 2nd, 3rd and 4th day after the induction of senescence.
Results. Eicosapentaenoic acid (50 µM) led to extensively decreased cell growth in peroxide induced senescence and decrease of cell growth in etoposide induced senescence, however SA-b-galactosidase activity, p21 protein expression, and the amount of reactive oxygen species in cells did not significantly increase in either peroxide induced senescence or in etoposide induced senescence. In peroxide induced senescence caspase-3 protein expression or apoptotic DNA cleavage were not detected.
Conclusion. Increased formation of senescent cells in peroxide or etoposide induced senescence or apoptosis in peroxide induced senescence are probably not a cause of the reduced cell growth after the postreatment with eicosapentaenoic acid (50 µM) (Fig. 6, Ref. 17). Text in PDF www.lekarskyobzor.sk.
KEY WORDS: senescence, omega-3 fatty acids, eicosapentaenoic acid, MRC-5 human lung fibroblasts, aging.
Lek Obz 2022, 71 (11): 491-497
Mária JANUBOVA, Katarína KOŇARIKOVÁ, Ingrid ŽITŇANOVÁ
Institute of Medical Chemistry, Biochemistry and Clinical Biochemistry, Faculty of Medicine, Comenius University, Bratislava, head of the institute prof. MUDr. L. Turecký, PhD.
CITE
JANUBOVA M., KOŇARIKOVÁ K., ŽITŇANOVÁ I.: Effect of eicosapentaenoic acid on cells growing in senescence inducing conditions. Lek Obz 2022, 71 (11): 491-497
