SUMMARY
Introduction: Based on several scientific works, the connection between selected microRNA-375, microRNA-124a, microRNA-29a, microRNA-33a, microRNA-9, microRNA-15a and the pathogenesis of diabetes mellitus is proven. In our pilot study, we attempted to evaluate the potential of using serum microRNA levels as biomarkers of clinically significant hypoglycemia.
Study design and methods: At specified intervals, serum samples were collected from 22 patients with diabetes mellitus after an episode of clinically significant hypoglycemia according to ADA 2021. Of the 109 samples collected, 654 Ct (cycle threshold) values of selected microRNAs were obtained by Real Time PCR and subsequently analysed and compared with patient glycemia, acquired at the identical time of serum collection.
Results: Our study did not confirm a significant correlation between blood glucose levels and selected microRNAs. We have observed a statistically significant negative correlation of microRNA-375 and microRNA-29a levels with the patient‘s age, also a significant negative correlation of microRNA-33a levels with BMI. In contrast, a positive correlation of microRNA-124a with BMI was shown. A significant trend was displayed by the positive correlation of microRNA-29a and microRNA-15a with eGFR and microRNA-375 with diabetes mellitus duration.
Conclusion: Although microRNAs appear to be a promising marker for the monitoring and diagnosis of several chronic diseases, the potential of their use in monitoring sudden conditions such as hypoglycemia has not been confirmed, presumably due to their complex biological mechanism and multifactorial regulation.
Key words: biomarker, hypoglycemia, microRNA, diabetes mellitus.
Lek Obz, 2021, 70 (9): 310-315
Veronika MIKUŠOVÁ, Ján MIKUŠ, Kristína GRILUSOVÁ, Lukáš URBAN, Mariana RONČÁKOVÁ, Emil MARTINKA
National Institute of Endocrinology and Diabetology n.o. in Ľubochňa, head doc. MUDr. E. Martinka PhD.
Cite:
MIKUŠOVÁ V., MIKUŠ J., GRILUSOVÁ K., URBAN L., RONČÁKOVÁ M., MARTINKA E.: MicroRNAs as biomarkers of clinically significant hypoglycemia. Lek Obz, 2021, 70 (9): 310-315
