SUMMARY
Introduction: Fecal microbiota transplantation (FMT) is currently a common treatment for Clostridioides difficile infection, and clinical studies are underway on the use of FMT in inflammatory bowel disease. The mechanism of the effect of FMT on the dysbiotic microbiota of patients has not yet been elucidated.
Methods: We studied changes in the stabilized gut microbiota of a patient with ulcerative colitis (female, 27, severe ulcerative colitis - mayo score 11) using an in vitro model of the human gastrointestinal tract - SHIME®. After stabilization of the gut microbiota, FMT of a healthy donor (male, 35) was applied to the model, totally twice within three days. Samples were withdrawn from the model for PCR-DGGE analysis, quantitative PCR, and sequencing of the bacterial 16S rRNA metagenome (NGS). Sequencing data were processed in Microsoft Excel.

Results: PCR-DGGE analyzes showed an observable effect of FMT on the gut microbiota by increasing bacterial diversity. Sequencing data confirmed and clarified the observations of PCR-DGGE analyzes.
Conclusions: Our work provided information on the changes induced by FMT on the gut microbiota observed in experiments using in vitro SHIME® model.
Key words: gut microbiota, ulcerative colitis, in vitro SHIME® model, fecal microbiota transplantation, next generation sequencing.
Lek Obz, 2021, 70(10): 350-355

René LINK 1, Anna KAMLÁROVÁ 1, Ľuboš AMBRO 1, Adriána LIPTÁKOVÁ 2

1 Ústav experimentálnej medicíny, Lekárska fakulta Univerzita Pavla Jozefa Šafárika, Košice, vedúca RNDr. I. Bertková, PhD.
2 Mikrobiologický ústav Lekárskej fakulty UK a UNB, Bratislava, prednostka doc. MUDr. A. Liptáková, PhD.

Cite:
LINK R., KAMLÁROVÁ A., AMBRO Ľ., LIPTÁKOVÁ A.: Pilot study of the effect of fecal transplantation on a gut modulated in an in vitro  SHIME® model. Lek Obz, 2021, 70(10): 350-355